Automated solid-phase microextraction and thin-film microextraction for high-throughput analysis of biological fluids and ligand-receptor binding studies.

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dc.contributor.authorVuckovic, Dajana
dc.contributor.authorCudjoe, Erasmus
dc.contributor.authorMusteata, Florin Marcel
dc.contributor.authorPawliszyn, Janusz
dc.date.accessioned2025-02-26T20:17:25Z
dc.date.available2025-02-26T20:17:25Z
dc.date.issued2010-01-07
dc.descriptionClick on the resource link to view the article (may not be free).
dc.description.abstractThis protocol describes how to perform automated solid-phase microextraction (SPME) and thin-film microextraction (TFME) in a 96-well plate format for high-throughput analysis of drugs, metabolites and any other analytes of interest in biological fluids using liquid chromatography-electrospray tandem mass spectrometry. Sample preparation time required is typically 1 min per sample; hence, the throughput achievable with automated SPME/TFME is comparable with automated 96-well liquid-liquid extraction and solid-phase extraction methods, but greater than most online solid-phase extraction methods. The technique is applicable to complex samples such as whole blood without additional pretreatment. The amount of analyte extracted by SPME/TFME is proportional to the free (unbound) concentration of the analyte; hence, SPME/TFME can be used to determine both total and free concentrations of analytes from a single biofluid sample and to perform automated ligand-receptor binding studies in order to determine binding affinity and/or overall extent of ligand binding to a complex biofluid.
dc.description.urihttps://doi.org/10.1038/nprot.2009.180
dc.identifier.citationVuckovic D, Cudjoe E, Musteata FM, Pawliszyn J. Automated solid-phase microextraction and thin-film microextraction for high-throughput analysis of biological fluids and ligand-receptor binding studies. Nat Protoc. 2010 Jan;5(1):140-61. doi: 10.1038/nprot.2009.180. Epub 2010 Jan 7. PMID: 20057385.
dc.identifier.doi10.1038/nprot.2009.180
dc.identifier.issn1750-2799
dc.identifier.other20057385
dc.identifier.piinprot.2009.180
dc.identifier.pmid20057385
dc.identifier.urihttps://hdl.handle.net/20.500.14303/899
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.ispartofNature Protocols
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). http://rightsstatements.org/vocab/InC/1.0/
dc.source.beginpage140
dc.source.countryEngland
dc.source.endpage161
dc.source.issue1
dc.source.journaltitleNature protocols
dc.source.volume5
dc.subjectBlood Chemical Analysis / methods
dc.subjectSolid Phase Microextraction / methods
dc.subjectUrine
dc.titleAutomated solid-phase microextraction and thin-film microextraction for high-throughput analysis of biological fluids and ligand-receptor binding studies.
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
local.departmentprogramDepartment of Pharmaceutical Sciences
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