Development of supported liquid-phase microextraction probes for in vivo PK studies.
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Issue Date
2015
Authors
Donabella, Paul J.*
Rogers, Nathan*
Levin, Robert M
Musteata, Florin Marcel
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Abstract
Background: A new sample preparation method termed supported liquid-phase microextraction is proposed. With this technique, the extraction phase is a liquid immobilized inside the pores of a membrane coated on a solid support.
Methodology: Supported liquid-phase microextraction probes were prepared by coating wires with porous polyacrylonitrile followed by saturation with 1-octanol. The probes were introduced inside hypodermic needles and used for in vivo extraction of oxybutynin from the blood and tissues of rabbits. The linear range of the method was from 0.5 to 500 ng/ml.
Conclusion: The proposed method was successfully applied to monitor the PK profile of oxybutynin. The drug followed a two-compartment model, with a volume of distribution of 14 l/kg and a half-life of 76 min.
Citation
Donabella P, Rogers N, Levin R, Musteata FM. Development of supported liquid-phase microextraction probes for in vivo PK studies. Bioanalysis. 2015;7(6):661-70. doi: 10.4155/bio.15.7. PMID: 25871585.
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