Effects of Helicobacter pylori on Gastric and Colon Tumor Growth and Tumor Angiogenesis
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Issue Date
2014
Authors
Ghoth, Shahad '14
Degree
MS in Biotechnology
Advisor
Stein, Markus
Mousa, Shaker
Mousa, Shaker
Committee Members
Thangirala, Sudha
Journal Title
Journal ISSN
Volume Title
Abstract
Helicobacter pylori (H. pylori) is a human pathogen that is associated with severe gastric diseases including gastritis and gastric cancer. H. pylori expresses many virulence factors such as the cytotoxin-associated gene A (CagA). CagA induces many cancer-related host-signaling events and appears to be the most important bacterial factor for the development of gastric adenocarcinoma. However, the bacterial disease progression depends also on many additional factors including genetic predisposition of the host and environmental factors. Another important process that is required for cancer development is angiogenesis. While some studies have stated that H. pylori can promote angiogenesis, other studies have indicated that H. pylori causes inhibition of endothelial proliferation. To examine these somehow conflicting results in more detail, a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the effect of H. pylori on cell growth. In addition, a chicken chorioallantoic membrane (CAM) and a nude mouse model were used to further investigate how H. pylori affects angiogenesis and tumor growth. The CAM model results did not show any effect of H. pylori on cancer cells. However, the in vitro model and the mouse model showed that H. pylori has a significant effect on the growth of AGS and Colo205 cancer cells. In conclusion, H. pylori strain G27 promotes the growth of cancer cell line AGS.
Citation
Ghoth, Shahad. "Effects of Helicobacter Pylori on Gastric and Colon Tumor Growth and Tumor Angiogenesis." Albany College of Pharmacy and Health Sciences, New York, Proquest/UMI, 2014.
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