Measurement of free fraction, total concentration and protein binding for testosterone, triiodothyronine and thyroxine.

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Issue Date

10/17/2023

Authors

Klock, Emily*
Kane, Michael P
Musteata, Florin M

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Abstract

Measuring the total and free concentrations of hormones is useful, but the technology to do this simultaneously is lacking. A new method offers the ability to measure these parameters concurrently for testosterone, thyroxine and triiodothyronine. The free concentrations showed significant correlations with patients' vital statistics. Overall, 67% of correlations for total concentration showed that the new and classical methods had equal accuracy, or that comprehensive ultrafiltration was more accurate. The protein binding term was found to correlate significantly with the patients' luteinizing hormone, prostate-specific antigen and height. Comprehensive ultrafiltration for measuring the total concentration, free concentration and protein binding term uses less sample and is much faster than measuring these parameters with three separate methods.

Citation

Klock E, Kane MP, Musteata FM. Measurement of free fraction, total concentration and protein binding for testosterone, triiodothyronine and thyroxine. Bioanalysis. 2023 Oct 17. doi: 10.4155/bio-2023-0126. Epub ahead of print. PMID: 37847058.

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Grants

1R15GM126510/GM/NIGMS NIH HHS/United States