Transdermal sampling of vitamin D and 25-hydroxyvitamin D.
Loading...
Issue Date
2019-01
Authors
Mall, Isaac*
Musteata, Florin Marcel
Degree
Advisor
Committee Members
Journal Title
Journal ISSN
Volume Title
Abstract
Aim: Transdermal analysis is proposed for vitamin D3 and its hydroxylated metabolite to overcome problems associated with blood analysis.
Methods: Vitamin D3 was extracted directly from skin with solid patches and liquid phases. Deuterium-labeled vitamin D3 was added to the extraction solutions to compensate for variability and accurately determine the rate of transdermal transfer. Of the different extraction solvents tested, 50:50 octanol:isopropanol showed the best results, with an accuracy of 115% and reproducibility better than 30%.
Conclusion: The research shows that transdermal route can be used for analysis of vitamin D3 in porcine skin. When microneedles are used, accurate measurements were obtained in 1 h. With intact skin, the highest accuracy was obtained when extraction was done for 2 h.
Citation
Mall I, Musteata FM. Transdermal sampling of vitamin D3 and 25-hydroxyvitamin D3. Bioanalysis. 2019 Jan;11(2):61-72. doi: 10.4155/bio-2018-0138. Epub 2018 Nov 26. PMID: 30475076.
ACPHS Research Commons URI
Description
Click on the Resource Link to access the article (may not be free).