Vibrio parahaemolyticus infection stimulates pro-inflammatory cytokine production and innate lymphocyte cell activation
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Issue Date
2020-06
Authors
Dong, Chen Shu '20
Degree
MS in Molecular Biosciences
Advisor
Parent, Michelle A.
Committee Members
Shakerley, Nicole
Malik, Meenakshi
Yager, Eric
Malik, Meenakshi
Yager, Eric
Journal Title
Journal ISSN
Volume Title
Abstract
Vibrio parahaemolyticus, a Gram-negative bacillus, is the most common cause of bacterial seafood-related gastroenteritis in the United States. Infection is typically self-limiting; however, those with chronic medical conditions or the immunocompromised can be at risk for more severe infections leading to septicemia and death. At this time, in order understand and identify those essential parameters of infection survival, we are investigating the host immune response using a model of systemic infection. First, building upon previously published and established methods, we have documented systemic infection by demonstrating morbidity and dissemination by recoverable organisms isolated from spleen and liver. Additionally, systemic infection results in increased IL-6, TNF-a, and IL-12p70 proinflammatory cytokine production, along with increased C-reactive protein (CRP), a well-documented acute phase protein; all are associated with innate immunity. Interestingly, we determined early after infection, an unexpected increase in a CD8+NK1.1+CD69+ cell population, which is being further characterized to fully understand this cell types early role in combating infection with this pathogen. We have determined this CD8+NK1.1+CD69+ is an NKT-like cell that produces IFN-a and our preliminary data suggests a role as a short-lived effector cell (SLEC). Lastly, at day 3 post-infection we detected increased levels of Vibrio parahaemolyticus-specific IgM titers and at day 30 and day 60 post-infection increases in Vibrio parahaemolyticus-specific IgG antibody titers. This preliminary data allows us to begin to understand the innate and adaptive response required to eliminate this systemic and often life-threatening infection.
Citation
Dong CS. Vibrio parahaemolyticus infection stimulates pro-inflammatory cytokine production and innate lymphocyte cell activation [thesis]. Ann Arbor (MI): Proquest LLC; 2020. 93 p.
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