Monitoring of rat brain endocannabinoids using solid-phase microextraction.

Thumbnail Image
Issue Date
2019-08
Authors
Aslam, Momna
Feleder, Carlos
Newsom, Ryan J
Campeau, Serge
Musteata, Florin Marcel
Degree
Advisor
Committee Members
Journal Title
Journal ISSN
Volume Title
Abstract
Solid-phase microextraction is proposed to measure concentrations of anandamide and 2-arachidonoyl glycerol in live rat brains in response to stress. Solid-phase microextraction fibers were prepared from steel with 1.5 mm extraction coating. 24 male rats were divided into groups based on brain region, stria terminalis or posterior hypothalamus and loud noise or control groups. The fibers were desorbed in acetonitrile-water (75:25) and analyzed by ultraperformance LC-MS/MS. The linear range of the method was 0.05-50 ng/ml and the concentrations were found to be between 0.3 and 40 ng/ml. The new approach was successfully used to determine the concentrations of anandamide and 2-arachidonoyl glycerol and could be used in the future to measure other endogenous compounds.
Citation
Aslam M, Feleder C, Newsom RJ, Campeau S, Musteata FM. In vivo monitoring of rat brain endocannabinoids using solid-phase microextraction. Bioanalysis. 2019 Aug;11(16):1523-1534. doi: 10.4155/bio-2019-0144. Epub 2019 Sep 5. PMID: 31486681; PMCID: PMC6770421.
Description
Grants