Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR.
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Issue Date
9/25/2022
Authors
He, Peng
Faris, Shannon
Sagabala, Reddy Sudheer
Datta, Payel
Xu, Zihan
Callahan, Brian
Wang, Chunyu
Boivin, Benoit
Zhang, Fuming
Linhardt, Robert J
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Abstract
Cholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol-protein interactions in aqueous solutions is complicated by cholesterol's low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.
Citation
He P, Faris S, Sagabala RS, Datta P, Xu Z, Callahan B, Wang C, Boivin B, Zhang F, Linhardt RJ. Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR. Biosensors (Basel). 2022 Sep 25;12(10):788. doi: 10.3390/bios12100788. PMID: 36290926; PMCID: PMC9599816.
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Grants
R01 CA206592/CA/NCI NIH HHS/United States , T32 AG057464/AG/NIA NIH HHS/United States