Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR.

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dc.contributor.authorHe, Peng
dc.contributor.authorFaris, Shannon
dc.contributor.authorSagabala, Reddy Sudheer
dc.contributor.authorDatta, Payel
dc.contributor.authorXu, Zihan
dc.contributor.authorCallahan, Brian
dc.contributor.authorWang, Chunyu
dc.contributor.authorBoivin, Benoit
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J
dc.contributor.orcidhttps://orcid.org/0000-0001-5977-448X
dc.date.accessioned2024-05-08T13:46:57Z
dc.date.available2024-05-08T13:46:57Z
dc.date.issued9/25/2022
dc.description.abstractCholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol-protein interactions in aqueous solutions is complicated by cholesterol's low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.
dc.description.grantGrant Funded
dc.description.sponsorshipR01 CA206592/CA/NCI NIH HHS/United States
dc.description.sponsorshipT32 AG057464/AG/NIA NIH HHS/United States
dc.description.urihttps://doi.org/10.3390/bios12100788
dc.description.urihttp://www.ncbi.nlm.nih.gov/pmc/articles/pmc9599816/
dc.identifier.citationHe P, Faris S, Sagabala RS, Datta P, Xu Z, Callahan B, Wang C, Boivin B, Zhang F, Linhardt RJ. Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR. Biosensors (Basel). 2022 Sep 25;12(10):788. doi: 10.3390/bios12100788. PMID: 36290926; PMCID: PMC9599816.
dc.identifier.issn2079-6374
dc.identifier.other36290926
dc.identifier.urihttps://hdl.handle.net/20.500.14303/591
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofBiosensors
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). http://rightsstatements.org/vocab/InC/1.0/
dc.subjectbinding kinetics
dc.subjectbiotinylated cholesterol
dc.subjectcholesterol
dc.subjectcholesterol-binding proteins
dc.subjectsurface plasmon resonance
dc.titleCholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR.
dc.typeArticle
local.departmentprogramDepartment of Pharmaceutical Sciences
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