Stability and biodistribution of endocannabinoids across the human brain microvascular endothelium barrier
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Issue Date
2019-05
Authors
Pruyn, Schuyler '19
Degree
MS in Pharmaceutical Sciences
Advisor
Zheng, HaiAn
Committee Members
Voigt, Jeffrey M.
Musteata, F. Marcel
Musteata, F. Marcel
Journal Title
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Volume Title
Abstract
The discovery of the endocannabinoid system (ECS) has brought more interests for further research, which are essential to understand its mechanism of this system and the therapeutic potential of the cannabinoids and medical cannabis. The ECS is active throughout the central and peripheral nervous systems and is comprised of the cannabinoid receptors (CBRs), the endogenous ligands (endocannabinoids, eCBs), and their synthetic and metabolic enzymes. The endocannabinoids, such as anandamide (AEA) and 2-arachidonoylglyrol (2AG) are produced for many neurological responses, such as reducing sensitivity to pain. There is a need to develop simple, yet effective methods to identify and quantify these natural chemicals from various mediums and bio-matrices. Such real-time quantification will allow us to determine their chemical and biological distribution and metabolism when they circulate throughout the body. This study focuses on the stability of AEA and 2AG at blood-brain barrier (BBB) and their permeability across it. With properly developed methods, the chemical stability and kinetics, as well as the biodistribution across the BBB of these lipids are determined and quantified to better understand their clinical impacts.
Citation
Pruyn S. Stability and biodistribution of endocannabinoids across the human brain microvascular endothelium barrier [thesis]. Ann Arbor (MI): Proquest LLC; 2019. 76 p.
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