Investigating the host-pathogen interaction between Vibrio parahaemolyticus and macrophages, Understanding the role of reactive oxygen stresses

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dc.contributor.advisorParent, Michelle A.
dc.contributor.authorVojnar, Brittany '20
dc.contributor.committeememberShakerley, Nicole L.
dc.contributor.committeememberMalik, Meenakshi
dc.date.accessioned2023-04-18T18:59:12Z
dc.date.available2023-04-18T18:59:12Z
dc.date.issued2020-08
dc.descriptionClick on the Resource Link to find this item in the ACPHS Library catalog.
dc.description.abstractVibrio parahaemolyticus serovar O3:K6, the most common cause of bacterial seafood-related illness, is responsible for 48% of all reported Vibrio spp. infections in the United States. At this time, limited literature is available regarding organism pathogenesis and especially the host response and immunity to infection. It is well known that pathogenic bacteria may produce enzymes such as catalase (katE1, katE2), alkyl hydroperoxide reductase (ahpC1, ahpC2) and peroxiredoxins allowing for survival in toxic environments, specifically in the presence of reactive oxygen species (ROS). Toward that end, we have hypothesized that V. parahaemolyticus utilizes its antioxidant capabilities, such as katE, ahpC, and other antioxidant associated enzymes, to survive in diverse environments, not just in the mammalian host. Here, we investigated the antioxidant genes involved in V. parahaemolyticus survival when exposed to toxic oxygen species present in separate models; in-vitro culture and murine BMDM infection. We determined that catalases such as katE1 and katE2 and the peroxiredoxin and reductant system involving ahpC1 and ahpF are required for survival in different models of exposure to ROS. Additionally, these antioxidant genes are regulated by OxyR. At this time, characterization of organism survival in varying environments will allow for a more complete understanding of organism virulence and survival in different hosts and environments providing a comprehensive understanding of Vibrio parahaemolyticus pathogenesis. Ultimately, the goal is to formulate treatment algorithms to facilitate pathogenic organism removal regardless of the host or environment.
dc.description.degreeMS in Molecular Biosciences
dc.description.urihttps://acphs.on.worldcat.org/oclc/1294948002
dc.format.extent80 pages
dc.identifier.citationVojnar B. Investigating the host-pathogen interaction between Vibrio parahaemolyticus and macrophages, understanding the role of reactive oxygen stresses [thesis]. Ann Arbor (MI): ProQuest, LLC; 2020. 80 p.
dc.identifier.urihttps://hdl.handle.net/20.500.14303/30
dc.languageen
dc.publisherProQuest LLC
dc.relation.ispartofAlbany College of Pharmacy and Health Sciences Theses
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dc.subjectantioxidant
dc.subjectmacrophage
dc.subjectROS
dc.subjectVibrio parahaemolyticus
dc.titleInvestigating the host-pathogen interaction between Vibrio parahaemolyticus and macrophages, Understanding the role of reactive oxygen stresses
dc.typeThesis
local.departmentprogramDepartment of Life Sciences
local.departmentprogramMSMB MS in Molecular Biosciences
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