Determination of free and deconjugated testosterone and epitestosterone in urine using SPME and LC-MS/MS.

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dc.contributor.authorZhan, Yanwei
dc.contributor.authorMusteata, Florin M
dc.contributor.authorBasset, Fabien A
dc.contributor.authorPawliszyn, Janusz
dc.date.accessioned2025-02-27T15:34:11Z
dc.date.available2025-02-27T15:34:11Z
dc.date.issued2011-Jan
dc.descriptionClick on the resource link to view the article (may not be free).
dc.description.abstractBackground: A thin sheet of polydimethylsilosane membrane was used as an extraction phase for solid-phase microextraction. Compared with fiber or rod solid-phase microextraction geometries, the thin film exhibited much higher extraction capacity without sacrificing extraction time due to its higher area-to-volume ratio. The analytical method involved direct extraction of unconjugated testosterone (T) and epitestosterone (ET) followed by separation on a C18 column and detection by selected reaction monitoring in positive ionization mode.
dc.description.abstractResults: The limit of detection was 1 ng/l for both T and ET. After method validation, free (unconjugated) T and ET were extracted and quantified in real samples. Since T and ET are extensively metabolized, the proposed method was also applied to extract the steroids after enzymatic deconjugation of urinary-excreted steroid glucuronides.
dc.description.abstractConclusion: The proposed method allows quantification of both conjugated and unconjugated steroids, and revealed that there was a change in the ratio of T to ET after enzymatic deconjugation, indicating different rates of metabolism.
dc.description.urihttps://doi.org/10.4155/bio.10.177
dc.identifier.citationZhan Y, Musteata FM, Basset FA, Pawliszyn J. Determination of free and deconjugated testosterone and epitestosterone in urine using SPME and LC-MS/MS. Bioanalysis. 2011 Jan;3(1):23-30. doi: 10.4155/bio.10.177. PMID: 21175364.
dc.identifier.doi10.4155/bio.10.177
dc.identifier.issn1757-6199
dc.identifier.other21175364
dc.identifier.pmid21175364
dc.identifier.urihttps://hdl.handle.net/20.500.14303/900
dc.language.isoen
dc.publisherTaylor & Francis
dc.relation.ispartofBioanalysis
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). http://rightsstatements.org/vocab/InC/1.0/
dc.source.beginpage23
dc.source.countryEngland
dc.source.endpage30
dc.source.issue1
dc.source.journaltitleBioanalysis
dc.source.volume3
dc.subjectEpitestosterone
dc.subjectChromatography, High Pressure Liquid
dc.subjectSolid Phase Microextraction
dc.subjectTestosterone
dc.titleDetermination of free and deconjugated testosterone and epitestosterone in urine using SPME and LC-MS/MS.
dc.typeJournal Article
local.departmentprogramDepartment of Pharmaceutical Sciences
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